sequencing libraries Search Results


350 450 nucleotides  (Complete Genomics Inc)
96
Complete Genomics Inc 350 450 nucleotides
350 450 Nucleotides, supplied by Complete Genomics Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/350 450 nucleotides/product/Complete Genomics Inc
Average 96 stars, based on 1 article reviews
350 450 nucleotides - by Bioz Stars, 2026-05
96/100 stars
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mgieasy whole genome bisulfite library preparation kit  (Complete Genomics Inc)
97
Complete Genomics Inc mgieasy whole genome bisulfite library preparation kit
Figure 5. Genome-wide DNA methylation analysis in WT and GM seeds before and after GO treatments. a) Circos plots show the methylation levels across different chromosomes for WT and GM seeds before and after GO exposure. The colored sections on the outer ring represent different chromo- somes. The concentric rings from outer to inner illustrate untreated samples, 0.75 mg-C/L GO-treated samples, and 1.5 mg-C/L GO-treated <t>samples.</t> <t>Whole-genome</t> methylation levels were calculated as mean values within 100 kb windows for each chromosome. The color gradient from cyan to red indicates the methylation percentages, whereas the innermost ring represents gene density. b) Whole-genome methylation levels in CG, CHG, and CHH contexts for WT and GM seeds after various GO exposures (sample size n = 3). Data are presented as means ± SD, with gray and red dots depicting individual data points in the WT and GM groups. Three biological replicates were included for each treatment. Independent sample two-sided t-tests were performed: * indicates significant differences within the same genotype relative to the condition without GO, and # indicates significant differences between GM and WT under the same treatment. Significant differences are marked with p values. c) Differential methylation regions at promoter regions and gene bodies of key genes (Figure 1c) are represented by a color gradient showing Log2FC in methylation levels. Triangles and circles indicate CG and CHH methylation, respectively. No differential methylation was observed in the CHG context. Regions without differential methylation are blank. d) Dot plots of differential methylation for ALA metabolism-related genes compare promoter and gene body methylation across conditions. Blue and red dots denote different comparisons, as indicated in the legend. Dots with circles indicate methylation levels with |Log2FC| >10.
Mgieasy Whole Genome Bisulfite Library Preparation Kit, supplied by Complete Genomics Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mgieasy whole genome bisulfite library preparation kit/product/Complete Genomics Inc
Average 97 stars, based on 1 article reviews
mgieasy whole genome bisulfite library preparation kit - by Bioz Stars, 2026-05
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access array barcode library for illumina  (fluidigm)
95
fluidigm access array barcode library for illumina
Figure 5. Genome-wide DNA methylation analysis in WT and GM seeds before and after GO treatments. a) Circos plots show the methylation levels across different chromosomes for WT and GM seeds before and after GO exposure. The colored sections on the outer ring represent different chromo- somes. The concentric rings from outer to inner illustrate untreated samples, 0.75 mg-C/L GO-treated samples, and 1.5 mg-C/L GO-treated <t>samples.</t> <t>Whole-genome</t> methylation levels were calculated as mean values within 100 kb windows for each chromosome. The color gradient from cyan to red indicates the methylation percentages, whereas the innermost ring represents gene density. b) Whole-genome methylation levels in CG, CHG, and CHH contexts for WT and GM seeds after various GO exposures (sample size n = 3). Data are presented as means ± SD, with gray and red dots depicting individual data points in the WT and GM groups. Three biological replicates were included for each treatment. Independent sample two-sided t-tests were performed: * indicates significant differences within the same genotype relative to the condition without GO, and # indicates significant differences between GM and WT under the same treatment. Significant differences are marked with p values. c) Differential methylation regions at promoter regions and gene bodies of key genes (Figure 1c) are represented by a color gradient showing Log2FC in methylation levels. Triangles and circles indicate CG and CHH methylation, respectively. No differential methylation was observed in the CHG context. Regions without differential methylation are blank. d) Dot plots of differential methylation for ALA metabolism-related genes compare promoter and gene body methylation across conditions. Blue and red dots denote different comparisons, as indicated in the legend. Dots with circles indicate methylation levels with |Log2FC| >10.
Access Array Barcode Library For Illumina, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/access array barcode library for illumina/product/fluidigm
Average 95 stars, based on 1 article reviews
access array barcode library for illumina - by Bioz Stars, 2026-05
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miseq sequencers  (fluidigm)
92
fluidigm miseq sequencers
Figure 5. Genome-wide DNA methylation analysis in WT and GM seeds before and after GO treatments. a) Circos plots show the methylation levels across different chromosomes for WT and GM seeds before and after GO exposure. The colored sections on the outer ring represent different chromo- somes. The concentric rings from outer to inner illustrate untreated samples, 0.75 mg-C/L GO-treated samples, and 1.5 mg-C/L GO-treated <t>samples.</t> <t>Whole-genome</t> methylation levels were calculated as mean values within 100 kb windows for each chromosome. The color gradient from cyan to red indicates the methylation percentages, whereas the innermost ring represents gene density. b) Whole-genome methylation levels in CG, CHG, and CHH contexts for WT and GM seeds after various GO exposures (sample size n = 3). Data are presented as means ± SD, with gray and red dots depicting individual data points in the WT and GM groups. Three biological replicates were included for each treatment. Independent sample two-sided t-tests were performed: * indicates significant differences within the same genotype relative to the condition without GO, and # indicates significant differences between GM and WT under the same treatment. Significant differences are marked with p values. c) Differential methylation regions at promoter regions and gene bodies of key genes (Figure 1c) are represented by a color gradient showing Log2FC in methylation levels. Triangles and circles indicate CG and CHH methylation, respectively. No differential methylation was observed in the CHG context. Regions without differential methylation are blank. d) Dot plots of differential methylation for ALA metabolism-related genes compare promoter and gene body methylation across conditions. Blue and red dots denote different comparisons, as indicated in the legend. Dots with circles indicate methylation levels with |Log2FC| >10.
Miseq Sequencers, supplied by fluidigm, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/miseq sequencers/product/fluidigm
Average 92 stars, based on 1 article reviews
miseq sequencers - by Bioz Stars, 2026-05
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fragments 10 kbp and a library was constructed using a ligation sequencing kit  (Oxford Nanopore)
97
Oxford Nanopore fragments 10 kbp and a library was constructed using a ligation sequencing kit
Figure 5. Genome-wide DNA methylation analysis in WT and GM seeds before and after GO treatments. a) Circos plots show the methylation levels across different chromosomes for WT and GM seeds before and after GO exposure. The colored sections on the outer ring represent different chromo- somes. The concentric rings from outer to inner illustrate untreated samples, 0.75 mg-C/L GO-treated samples, and 1.5 mg-C/L GO-treated <t>samples.</t> <t>Whole-genome</t> methylation levels were calculated as mean values within 100 kb windows for each chromosome. The color gradient from cyan to red indicates the methylation percentages, whereas the innermost ring represents gene density. b) Whole-genome methylation levels in CG, CHG, and CHH contexts for WT and GM seeds after various GO exposures (sample size n = 3). Data are presented as means ± SD, with gray and red dots depicting individual data points in the WT and GM groups. Three biological replicates were included for each treatment. Independent sample two-sided t-tests were performed: * indicates significant differences within the same genotype relative to the condition without GO, and # indicates significant differences between GM and WT under the same treatment. Significant differences are marked with p values. c) Differential methylation regions at promoter regions and gene bodies of key genes (Figure 1c) are represented by a color gradient showing Log2FC in methylation levels. Triangles and circles indicate CG and CHH methylation, respectively. No differential methylation was observed in the CHG context. Regions without differential methylation are blank. d) Dot plots of differential methylation for ALA metabolism-related genes compare promoter and gene body methylation across conditions. Blue and red dots denote different comparisons, as indicated in the legend. Dots with circles indicate methylation levels with |Log2FC| >10.
Fragments 10 Kbp And A Library Was Constructed Using A Ligation Sequencing Kit, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fragments 10 kbp and a library was constructed using a ligation sequencing kit/product/Oxford Nanopore
Average 97 stars, based on 1 article reviews
fragments 10 kbp and a library was constructed using a ligation sequencing kit - by Bioz Stars, 2026-05
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nanopore libraries  (Oxford Nanopore)
96
Oxford Nanopore nanopore libraries
Figure 5. Genome-wide DNA methylation analysis in WT and GM seeds before and after GO treatments. a) Circos plots show the methylation levels across different chromosomes for WT and GM seeds before and after GO exposure. The colored sections on the outer ring represent different chromo- somes. The concentric rings from outer to inner illustrate untreated samples, 0.75 mg-C/L GO-treated samples, and 1.5 mg-C/L GO-treated <t>samples.</t> <t>Whole-genome</t> methylation levels were calculated as mean values within 100 kb windows for each chromosome. The color gradient from cyan to red indicates the methylation percentages, whereas the innermost ring represents gene density. b) Whole-genome methylation levels in CG, CHG, and CHH contexts for WT and GM seeds after various GO exposures (sample size n = 3). Data are presented as means ± SD, with gray and red dots depicting individual data points in the WT and GM groups. Three biological replicates were included for each treatment. Independent sample two-sided t-tests were performed: * indicates significant differences within the same genotype relative to the condition without GO, and # indicates significant differences between GM and WT under the same treatment. Significant differences are marked with p values. c) Differential methylation regions at promoter regions and gene bodies of key genes (Figure 1c) are represented by a color gradient showing Log2FC in methylation levels. Triangles and circles indicate CG and CHH methylation, respectively. No differential methylation was observed in the CHG context. Regions without differential methylation are blank. d) Dot plots of differential methylation for ALA metabolism-related genes compare promoter and gene body methylation across conditions. Blue and red dots denote different comparisons, as indicated in the legend. Dots with circles indicate methylation levels with |Log2FC| >10.
Nanopore Libraries, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nanopore libraries/product/Oxford Nanopore
Average 96 stars, based on 1 article reviews
nanopore libraries - by Bioz Stars, 2026-05
96/100 stars
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library preparation rapid sequencing  (Oxford Nanopore)
96
Oxford Nanopore library preparation rapid sequencing
Figure 5. Genome-wide DNA methylation analysis in WT and GM seeds before and after GO treatments. a) Circos plots show the methylation levels across different chromosomes for WT and GM seeds before and after GO exposure. The colored sections on the outer ring represent different chromo- somes. The concentric rings from outer to inner illustrate untreated samples, 0.75 mg-C/L GO-treated samples, and 1.5 mg-C/L GO-treated <t>samples.</t> <t>Whole-genome</t> methylation levels were calculated as mean values within 100 kb windows for each chromosome. The color gradient from cyan to red indicates the methylation percentages, whereas the innermost ring represents gene density. b) Whole-genome methylation levels in CG, CHG, and CHH contexts for WT and GM seeds after various GO exposures (sample size n = 3). Data are presented as means ± SD, with gray and red dots depicting individual data points in the WT and GM groups. Three biological replicates were included for each treatment. Independent sample two-sided t-tests were performed: * indicates significant differences within the same genotype relative to the condition without GO, and # indicates significant differences between GM and WT under the same treatment. Significant differences are marked with p values. c) Differential methylation regions at promoter regions and gene bodies of key genes (Figure 1c) are represented by a color gradient showing Log2FC in methylation levels. Triangles and circles indicate CG and CHH methylation, respectively. No differential methylation was observed in the CHG context. Regions without differential methylation are blank. d) Dot plots of differential methylation for ALA metabolism-related genes compare promoter and gene body methylation across conditions. Blue and red dots denote different comparisons, as indicated in the legend. Dots with circles indicate methylation levels with |Log2FC| >10.
Library Preparation Rapid Sequencing, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/library preparation rapid sequencing/product/Oxford Nanopore
Average 96 stars, based on 1 article reviews
library preparation rapid sequencing - by Bioz Stars, 2026-05
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sequencing library preparation  (Macrogen)
90
Macrogen sequencing library preparation
Figure 5. Genome-wide DNA methylation analysis in WT and GM seeds before and after GO treatments. a) Circos plots show the methylation levels across different chromosomes for WT and GM seeds before and after GO exposure. The colored sections on the outer ring represent different chromo- somes. The concentric rings from outer to inner illustrate untreated samples, 0.75 mg-C/L GO-treated samples, and 1.5 mg-C/L GO-treated <t>samples.</t> <t>Whole-genome</t> methylation levels were calculated as mean values within 100 kb windows for each chromosome. The color gradient from cyan to red indicates the methylation percentages, whereas the innermost ring represents gene density. b) Whole-genome methylation levels in CG, CHG, and CHH contexts for WT and GM seeds after various GO exposures (sample size n = 3). Data are presented as means ± SD, with gray and red dots depicting individual data points in the WT and GM groups. Three biological replicates were included for each treatment. Independent sample two-sided t-tests were performed: * indicates significant differences within the same genotype relative to the condition without GO, and # indicates significant differences between GM and WT under the same treatment. Significant differences are marked with p values. c) Differential methylation regions at promoter regions and gene bodies of key genes (Figure 1c) are represented by a color gradient showing Log2FC in methylation levels. Triangles and circles indicate CG and CHH methylation, respectively. No differential methylation was observed in the CHG context. Regions without differential methylation are blank. d) Dot plots of differential methylation for ALA metabolism-related genes compare promoter and gene body methylation across conditions. Blue and red dots denote different comparisons, as indicated in the legend. Dots with circles indicate methylation levels with |Log2FC| >10.
Sequencing Library Preparation, supplied by Macrogen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sequencing library preparation/product/Macrogen
Average 90 stars, based on 1 article reviews
sequencing library preparation - by Bioz Stars, 2026-05
90/100 stars
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library preparation and sequencing  (SeqMatic LLC)
90
SeqMatic LLC library preparation and sequencing
Figure 5. Genome-wide DNA methylation analysis in WT and GM seeds before and after GO treatments. a) Circos plots show the methylation levels across different chromosomes for WT and GM seeds before and after GO exposure. The colored sections on the outer ring represent different chromo- somes. The concentric rings from outer to inner illustrate untreated samples, 0.75 mg-C/L GO-treated samples, and 1.5 mg-C/L GO-treated <t>samples.</t> <t>Whole-genome</t> methylation levels were calculated as mean values within 100 kb windows for each chromosome. The color gradient from cyan to red indicates the methylation percentages, whereas the innermost ring represents gene density. b) Whole-genome methylation levels in CG, CHG, and CHH contexts for WT and GM seeds after various GO exposures (sample size n = 3). Data are presented as means ± SD, with gray and red dots depicting individual data points in the WT and GM groups. Three biological replicates were included for each treatment. Independent sample two-sided t-tests were performed: * indicates significant differences within the same genotype relative to the condition without GO, and # indicates significant differences between GM and WT under the same treatment. Significant differences are marked with p values. c) Differential methylation regions at promoter regions and gene bodies of key genes (Figure 1c) are represented by a color gradient showing Log2FC in methylation levels. Triangles and circles indicate CG and CHH methylation, respectively. No differential methylation was observed in the CHG context. Regions without differential methylation are blank. d) Dot plots of differential methylation for ALA metabolism-related genes compare promoter and gene body methylation across conditions. Blue and red dots denote different comparisons, as indicated in the legend. Dots with circles indicate methylation levels with |Log2FC| >10.
Library Preparation And Sequencing, supplied by SeqMatic LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/library preparation and sequencing/product/SeqMatic LLC
Average 90 stars, based on 1 article reviews
library preparation and sequencing - by Bioz Stars, 2026-05
90/100 stars
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library construction and sequencing for characterizing mrna, lncrna, and circrna expression  (Novogene)
90
Novogene library construction and sequencing for characterizing mrna, lncrna, and circrna expression
Figure 5. Genome-wide DNA methylation analysis in WT and GM seeds before and after GO treatments. a) Circos plots show the methylation levels across different chromosomes for WT and GM seeds before and after GO exposure. The colored sections on the outer ring represent different chromo- somes. The concentric rings from outer to inner illustrate untreated samples, 0.75 mg-C/L GO-treated samples, and 1.5 mg-C/L GO-treated <t>samples.</t> <t>Whole-genome</t> methylation levels were calculated as mean values within 100 kb windows for each chromosome. The color gradient from cyan to red indicates the methylation percentages, whereas the innermost ring represents gene density. b) Whole-genome methylation levels in CG, CHG, and CHH contexts for WT and GM seeds after various GO exposures (sample size n = 3). Data are presented as means ± SD, with gray and red dots depicting individual data points in the WT and GM groups. Three biological replicates were included for each treatment. Independent sample two-sided t-tests were performed: * indicates significant differences within the same genotype relative to the condition without GO, and # indicates significant differences between GM and WT under the same treatment. Significant differences are marked with p values. c) Differential methylation regions at promoter regions and gene bodies of key genes (Figure 1c) are represented by a color gradient showing Log2FC in methylation levels. Triangles and circles indicate CG and CHH methylation, respectively. No differential methylation was observed in the CHG context. Regions without differential methylation are blank. d) Dot plots of differential methylation for ALA metabolism-related genes compare promoter and gene body methylation across conditions. Blue and red dots denote different comparisons, as indicated in the legend. Dots with circles indicate methylation levels with |Log2FC| >10.
Library Construction And Sequencing For Characterizing Mrna, Lncrna, And Circrna Expression, supplied by Novogene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/library construction and sequencing for characterizing mrna, lncrna, and circrna expression/product/Novogene
Average 90 stars, based on 1 article reviews
library construction and sequencing for characterizing mrna, lncrna, and circrna expression - by Bioz Stars, 2026-05
90/100 stars
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library generation and sequencing  (Annoroad Gene Technology Co Ltd)
90
Annoroad Gene Technology Co Ltd library generation and sequencing
Figure 5. Genome-wide DNA methylation analysis in WT and GM seeds before and after GO treatments. a) Circos plots show the methylation levels across different chromosomes for WT and GM seeds before and after GO exposure. The colored sections on the outer ring represent different chromo- somes. The concentric rings from outer to inner illustrate untreated samples, 0.75 mg-C/L GO-treated samples, and 1.5 mg-C/L GO-treated <t>samples.</t> <t>Whole-genome</t> methylation levels were calculated as mean values within 100 kb windows for each chromosome. The color gradient from cyan to red indicates the methylation percentages, whereas the innermost ring represents gene density. b) Whole-genome methylation levels in CG, CHG, and CHH contexts for WT and GM seeds after various GO exposures (sample size n = 3). Data are presented as means ± SD, with gray and red dots depicting individual data points in the WT and GM groups. Three biological replicates were included for each treatment. Independent sample two-sided t-tests were performed: * indicates significant differences within the same genotype relative to the condition without GO, and # indicates significant differences between GM and WT under the same treatment. Significant differences are marked with p values. c) Differential methylation regions at promoter regions and gene bodies of key genes (Figure 1c) are represented by a color gradient showing Log2FC in methylation levels. Triangles and circles indicate CG and CHH methylation, respectively. No differential methylation was observed in the CHG context. Regions without differential methylation are blank. d) Dot plots of differential methylation for ALA metabolism-related genes compare promoter and gene body methylation across conditions. Blue and red dots denote different comparisons, as indicated in the legend. Dots with circles indicate methylation levels with |Log2FC| >10.
Library Generation And Sequencing, supplied by Annoroad Gene Technology Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/library generation and sequencing/product/Annoroad Gene Technology Co Ltd
Average 90 stars, based on 1 article reviews
library generation and sequencing - by Bioz Stars, 2026-05
90/100 stars
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library preparation and sequencing  (GenomeScan)
90
GenomeScan library preparation and sequencing
Figure 5. Genome-wide DNA methylation analysis in WT and GM seeds before and after GO treatments. a) Circos plots show the methylation levels across different chromosomes for WT and GM seeds before and after GO exposure. The colored sections on the outer ring represent different chromo- somes. The concentric rings from outer to inner illustrate untreated samples, 0.75 mg-C/L GO-treated samples, and 1.5 mg-C/L GO-treated <t>samples.</t> <t>Whole-genome</t> methylation levels were calculated as mean values within 100 kb windows for each chromosome. The color gradient from cyan to red indicates the methylation percentages, whereas the innermost ring represents gene density. b) Whole-genome methylation levels in CG, CHG, and CHH contexts for WT and GM seeds after various GO exposures (sample size n = 3). Data are presented as means ± SD, with gray and red dots depicting individual data points in the WT and GM groups. Three biological replicates were included for each treatment. Independent sample two-sided t-tests were performed: * indicates significant differences within the same genotype relative to the condition without GO, and # indicates significant differences between GM and WT under the same treatment. Significant differences are marked with p values. c) Differential methylation regions at promoter regions and gene bodies of key genes (Figure 1c) are represented by a color gradient showing Log2FC in methylation levels. Triangles and circles indicate CG and CHH methylation, respectively. No differential methylation was observed in the CHG context. Regions without differential methylation are blank. d) Dot plots of differential methylation for ALA metabolism-related genes compare promoter and gene body methylation across conditions. Blue and red dots denote different comparisons, as indicated in the legend. Dots with circles indicate methylation levels with |Log2FC| >10.
Library Preparation And Sequencing, supplied by GenomeScan, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/library preparation and sequencing/product/GenomeScan
Average 90 stars, based on 1 article reviews
library preparation and sequencing - by Bioz Stars, 2026-05
90/100 stars
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Image Search Results


Figure 5. Genome-wide DNA methylation analysis in WT and GM seeds before and after GO treatments. a) Circos plots show the methylation levels across different chromosomes for WT and GM seeds before and after GO exposure. The colored sections on the outer ring represent different chromo- somes. The concentric rings from outer to inner illustrate untreated samples, 0.75 mg-C/L GO-treated samples, and 1.5 mg-C/L GO-treated samples. Whole-genome methylation levels were calculated as mean values within 100 kb windows for each chromosome. The color gradient from cyan to red indicates the methylation percentages, whereas the innermost ring represents gene density. b) Whole-genome methylation levels in CG, CHG, and CHH contexts for WT and GM seeds after various GO exposures (sample size n = 3). Data are presented as means ± SD, with gray and red dots depicting individual data points in the WT and GM groups. Three biological replicates were included for each treatment. Independent sample two-sided t-tests were performed: * indicates significant differences within the same genotype relative to the condition without GO, and # indicates significant differences between GM and WT under the same treatment. Significant differences are marked with p values. c) Differential methylation regions at promoter regions and gene bodies of key genes (Figure 1c) are represented by a color gradient showing Log2FC in methylation levels. Triangles and circles indicate CG and CHH methylation, respectively. No differential methylation was observed in the CHG context. Regions without differential methylation are blank. d) Dot plots of differential methylation for ALA metabolism-related genes compare promoter and gene body methylation across conditions. Blue and red dots denote different comparisons, as indicated in the legend. Dots with circles indicate methylation levels with |Log2FC| >10.

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Accounting for ALA Natural Mutations Enhances the Efficiency of Graphene Oxide Nanopriming in Bar-Modified Arabidopsis.

doi: 10.1002/advs.202500058

Figure Lengend Snippet: Figure 5. Genome-wide DNA methylation analysis in WT and GM seeds before and after GO treatments. a) Circos plots show the methylation levels across different chromosomes for WT and GM seeds before and after GO exposure. The colored sections on the outer ring represent different chromo- somes. The concentric rings from outer to inner illustrate untreated samples, 0.75 mg-C/L GO-treated samples, and 1.5 mg-C/L GO-treated samples. Whole-genome methylation levels were calculated as mean values within 100 kb windows for each chromosome. The color gradient from cyan to red indicates the methylation percentages, whereas the innermost ring represents gene density. b) Whole-genome methylation levels in CG, CHG, and CHH contexts for WT and GM seeds after various GO exposures (sample size n = 3). Data are presented as means ± SD, with gray and red dots depicting individual data points in the WT and GM groups. Three biological replicates were included for each treatment. Independent sample two-sided t-tests were performed: * indicates significant differences within the same genotype relative to the condition without GO, and # indicates significant differences between GM and WT under the same treatment. Significant differences are marked with p values. c) Differential methylation regions at promoter regions and gene bodies of key genes (Figure 1c) are represented by a color gradient showing Log2FC in methylation levels. Triangles and circles indicate CG and CHH methylation, respectively. No differential methylation was observed in the CHG context. Regions without differential methylation are blank. d) Dot plots of differential methylation for ALA metabolism-related genes compare promoter and gene body methylation across conditions. Blue and red dots denote different comparisons, as indicated in the legend. Dots with circles indicate methylation levels with |Log2FC| >10.

Article Snippet: Library construction was performed using an MGIEasy whole-genome bisulfite library preparation kit (MGI).

Techniques: Genome Wide, DNA Methylation Assay, Methylation